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Before reverse transcription, ribosomal RNA was removed using Ribo-Zero rRNA removal kit (Bacteria; Illumina, USA).
Finally, the tungsten mask was removed using H2O2 (30%).
Contaminating genomic DNA was removed using a Qiagen RNase free DNase set.
Genomic DNA was removed using an RNase-free DNase kit (QIAGEN).
After 10 min, excess solution was removed using a strip of filter paper.
The supernatant was removed using a pipette and filtered to remove sediment particles.
Excess liquid was removed using a transfer pipette fitted with a 200-μL pipette tip.
Free dye was removed using Zeba Spin desalting columns with 7K MWCO (Thermo Fisher Scientific).
Residual DNA in the samples was removed using DNase I (Takara).
In this study, vanadium was removed using a new nanocomposite adsorbent called PdO-MWCNTs.
Then free DNA was removed using centrifugation.
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