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Approximately 0.7 ml of blood was collected by cardiac puncture into non-heparinized syringes and immediately decanted into serum gel S/1.3 tubes (Sarstedt, Nürnbrecht, Germany), after which the heart was removed to terminate the experiment.
At the end of 1 hour of resuscitation, an i.v. infusion of Ringer's lactate (1.5 ml/kg/hour) was started as fluid replacement and maintained throughout the experiment for a further 3 hours, at which point 1.2-ml blood samples were collected via the carotid artery into S/1.3 tubes containing serum gel (Sarstedt, Numbrecht, Germany), after which the heart was removed to terminate the experiment.
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At each time point (5, 20 or 60 min), 5 μl of the reaction mixture was removed and terminated by the addition of Laemmli sample buffer and boiled for 5 min.
Media was removed from each well to terminate cell treatments.
If the ICP device was removed prior to the sixth day, data collection was terminated.
DNA from the cosmid clones was digested with EcoRI and at 10 min intervals a 10 ml aliquot of the digest was removed and further digestion terminated by the addition of 1 ml of 0.5 M EDTA.
The LMA was removed, and ventilator support was terminated.
After 4 hours the medium was removed and the reaction was terminated with the addition of 100 μL dimethyl sulfoxide (DMSO) as a solubilization buffer to each well to dissolve the formazan crystals.
The terminating stop codon was removed before training so that stop codons would not be inserted into our artificial genome in frame, as this does not occur biologically and would bias the calculation of the expected frequency of OSCs.
First, Si (111) substrate was cleaned in the standard RCA cleaning process, then the native oxide of the Si substrate surface was removed and the Si surface was terminated with hydrogen by a diluted hydrogen fluoride solution (HF; H2O = 1 100).
Neilson said when Oponyo was removed from her position and the project terminated, she issued "a series of wild demands and threats.
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CEO of Professional Science Editing for Scientists @ prosciediting.com