Sentence examples for was removed by adding from inspiring English sources

The His-tag was removed by adding thrombin protease in to the protein solution at 1 100 (by mass) and incubated at 4 °C overnight.

Immediately following the RT reaction, RNA was removed by adding 2.5 U RNase H (New England Biolabs) and incubating the reaction at 37 °C for 20 min; the RNAse enzyme was subsequently inactivated by heating to 65 °C for 15 min.

The RNA template was removed by adding 1 µL of RNase H (Invitrogen) and incubating at 37°C for 20 min, 75°C for 15min and cooling to 4°C.

28 μm of metal was ground away by using a 60# grit size abrasive; and 20 μm more in thickness was removed by adding one more step of the grinding process using the 180# grit size abrasive.

SPS (sodium polyanetholesulfonate) was removed by adding benzyl alcohol to the solution and subsequently separated from the aqueous phase containing the DNA by centrifugation.

The starting RNA was removed by adding 0.5 µl RNaseH (2 U/µl) and heating at 37°C for 20 min. Samples were subsequently stored at −20°C.

After removal of the supernatant, red blood cells were removed by adding and suspending in 10-fold volume of sterile distilled water.

In polymerase chain reaction (PCR) RNase activity is removed by adding inhibitors.

Numerical instability caused by the tension-only membrane has been removed by adding an artificial shell with small stiffness.

Other impurities, collectively called gangue, are removed by adding a flux with which they combine to form a slag.

Iron, silica, and similar impurities that form colloidal hydroxides are removed by adding a small amount of activated carbon and digesting for one to two hours.