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The results showed that CK18 Asp396 was released from cell and tissue cultures to the extracellular compartment and that the protein complexes were stable, suggesting that CK18-based assays would be applicable in clinical studies.
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Culture supernatants were also collected to see if PrPSc was released from cells.
Virus was released from cells by multiple rounds of freezing and thawing and titered on permissive BHK cells or BSC40 cells by plaque staining assays.
Virus was released from cells by freeze/thaw cycles.
Poly(A)′-tailed mRNAs are released from cell lysate, captured by oligo-dT primers, and made into complementary DNAs (cDNAs) using a highly sensitive reverse transcription method.
Within minutes of systemin perception, the cytosolic Ca2+ concentration increases, and linolenic acid is released from cell membranes after a phospholipase has been activated.
Arachidonic acid is released from cell membranes by the action of phospholipases.
Additionally, tumor antigens that are released from cell lysis may be recognized and processed by immune cells.
Arachidonic acid is released from cell membranes by inflammatory stimuli and oxidized into compounds such as thromboxanes.
Increased total GAGs in medium with high glucose treatment suggest that GAGs are released from cell proteoglycan core proteins.
Cells cannot proliferate, when they are released from cell cycle arrest by removing CDKN1A (p21) and CDKN2A (p16) or by washing out DNA damaging drugs and CDK inhibitors.
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