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Methylation of more than 15% was regarded indicative for promoter hypermethylation.
A ratio of >1.3 was regarded indicative for gene copy number gain and a ratio >2.0 for gene amplification.
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Our results should be regarded indicative for those factors that were found to increase the risk for PWD.
Only the probability P ≤ 5% was regarded as indicative of statistical significance.
A p value <0.05 was regarded as indicative of statistical significance.
A promoter methylation ratio > 0.15 (corresponding to > 15% methylation) was regarded as indicative for promoter hypermethylation, based on cell-line experiments and previous experience [ 4, 18].
If the CK level had not decreased to normal levels, a second increase of more than 200 IU per litre over the previous value was regarded as indicative of a recurrent infarction [ 19].
In brief, heterodisomy for at least one locus was regarded as indicative of TR- or GC-mediated UPD 14 pat (TR/GC subtype), whereas isodisomy for all the informative microsatellite loci was interpreted as indicative of MR- or PE-mediated UPD 14 pat (MR/PE subtype) (for details, see Supplementary Figure S1).
E. coli was excreted by more than half of the diarrhoeic calves, but since this organism is regarded as a normal member of the intestinal flora of warm blooded animals, the finding of E. coli as such was regarded as indicative of a normal flora.
An ECG with persistent or transient ST-segment, new horizontal or down-sloping ST depression ≥0.05 mV in two contiguous leads and/or T inversion ≥0.1 mV in two contiguous leads with prominent R-wave or R/S ratio >1 was regarded as indicative of acute myocardial ischaemia and therefore registered as positive; all other cases were registered as negative[ 13].
Staining of ≥3+ (≥3 g/L) was regarded as proteinuria indicative of overt nephritis.
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