The purified protein was reconstituted into phospholipid vesicles and its transport properties and kinetic parameters were characterized.
SEC separated rfhSP-D protein was reconstituted into 100 mM ammonium acetate, pH 7.5 using biospin buffer exchange columns (Bio-Rad Laboratories).
Photo-reactive DNA was reconstituted into nucleosomes and bound with saturating amounts of wild type or mutant INO80 at 30 °C for 30 min.
The SaTRIC, purified in detergent DM, was reconstituted into the LCP by mixing with monoolein at a 2 3 protein to lipid ratio (w/w), using the twin-syringe mixing method.
When this nanopore was reconstituted into a synthetic planar lipid bilayer, addition of poly ethylene glycols) produced modifications in the single-channel conductance, allowing for the evaluation of the nanopore diameter.
The tubes were allowed to cool to room temperature and the derivatised compound was reconstituted into 80 μL of chloroform.
Five milligrams of nanoparticle solid was reconstituted into 5 ml of deionized water and then introduced into a dialysis tubing.
Purified lac permease, the 46.5-kDa product of the lac Y gene that catalyzes lactose/H+ symport, or purified cytochrome o, a terminal oxidase of the Escherichia coli respiratory chain composed of four subunits with a composite molecular mass of 140 kDa, was reconstituted into proteoliposomes individually or in combination.
The purified mAQP4 M23 was reconstituted into liposomes by following a previously published protocol [25].
Omp36 was purified using ion-exchange chromatography and a single trimeric porin was reconstituted into artificial lipid membranes [17], [23].
A shift to lower wavelength values was always observed when the protein was reconstituted into lipid membranes (representative spectra showed with the E. coli membranes in Figure 5B).
