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The Hessian fly was reared using methods described in [ 92].
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To test the hypothesis, a total of 2931 rainbow trout from 210 families were reared using two diets in a split-family design to weight of 2.5 2.7 kg.
RKC were reared using four temperature treatments ranging from 1.5° to 12 °C for a period of 60 days, both individually and in low-density populations.
Mosquitoes were reared using standard laboratory procedures [24].
Throughout the world the majority of broilers are reared using very similar, modern, intensive systems of production where birds are confined for their lifetime within high density housing [4] and reared from hatch to slaughter weight within approximately 40 days.
D. simulans flies infected with wAu from Coffs Harbour, Australia, were reared using standard techniques.
I.scapularis ticks were reared using standard methods [ 36]. Larval ticks were blood fed on hamsters.
Mosquitoes for this study were reared using techniques and conditions described in Aryan et al. (2013).
All fish species are reared using classic selection methods, without genotype modification.
Aedes aegypti were reared using standard procedures to either 0 2 days (young) or 14 16 days (old) post-eclosion before collection.
Once successfully genotyped, the freshly hatched An. coluzzii broods (first instar larvae) were reared using a standard larval rearing protocol (see above).
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