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While GCase mRNA was readily amplified from total or positive (DRBP76) IP samples, no significant amplification was observed in mouse isotype-matched IgG controls (Fig. 3C).
Confirming specificity of the approach, no PCR product was obtained with O51C/O52C when the Fbxl3fl expression vector was used as a template while the expected 474 nt product was readily amplified from the Fbxl3sv expression construct.
By contrast, actin was readily amplified in all tissues.
Therefore, DNA extracted using the TCM-CTAB method was readily amplified compared to the other three kits.
The amplicon C was readily amplified from chromatins in anti-p53 immunoprecipitates but not in control immunoglobulin G immunoprecipitates.
Although rMSP5 was readily amplified in parasites from asymptomatic and symptomatic infections, the antibody response was restricted to the asymptomatic group.
Similar(51)
First, many genes were readily amplified and sequenced with this method (29/47), and secondly introns were absent in the amplified portion of the gene about half of the time (15 /29).
Signal DNA molecules released then can be readily amplified with LAMP method.
Single-copy genomic sequences were amplified by a factor of more than 10 million with very high specificity, and DNA segments up to 2000 base pairs were readily amplified.
Samples which could not be readily amplified using standard protocols were amplified with the high-fidelity enzyme Herculase® (Stratagene, La Jolla, CA), using manufacturer supplied protocols.
All 7 meningococcal housekeeping genes were readily amplified during MLST, suggesting that M16160 is indeed Nm ST-74566, cc60), and again showing sodC to be a useful tool for definitive identification of carriage isolates.
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