Sentence examples for was randomly integrated from inspiring English sources

Southern blot analysis of transformants indicated that the plasmid vector was randomly integrated in multiple copies into the genome of C. acutatum.

Another "Super Pgap" mutant library was further constructed where the strong native promoter Pgap of Z. mobilis was randomly integrated throughout the genome of Z. mobilis 33C via an in vitro transposon mutagenesis system.

The efe gene was randomly integrated into the genome and expressed by a variety of promoters, and resultant strains were screened for the highest rates of production.

To achieve this task, a promoterless gene encoding a chloramphenicol acetyltransferase (cat), was randomly integrated into the S. gordonii chromosome and transformants were selected for chloramphenicol resistance.

8) How long are the Smu and Salpha sequences described in Figure 8? 9) The fact that the cassette was randomly integrated into CH12 cells and that the results were variable makes it difficult to interpret the results of this experiment.

To test this hypothesis we performed microarray experiments with either treated or untreated AX2 wt cells (wt; experiment I), the STATc knock-out mutant (STATc-, experIIInt III) and a mutant with an isogenic background where the STATc knock-out construct was randomly integrated into the genome (RIC, experiment II) (Table 3).

For example, site-specific insertion of cassettes can be carried out in preliminary randomly integrated recombinogenic sites [ 3], or the "marked" cassettes can be randomly integrated, followed by excision of the marker by a site-specific recombination system [ 1].

This can be expected because the construct carrying the genes is randomly integrated in the genome of A. niger and its copy number varies.

Building upon these earlier attempts, in the present work we improved transformation conditions through the development of two new vectors and also gained insight into the fate of the transferred T-DNA, which was found to be randomly integrated, with a preference for repeat element-containing genome sites.

Since the Cre cDNA has been randomly integrated into the genomic DNA [11], it is likely that not all copies integrated into the genome have the same transcriptional activity.

In most cases, multiple copies of the transgene, arrayed in a head-to-tail manner, are randomly integrated in the host genome, which may cause transcriptional interference that represses expression [15] [17].