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Cis retinoic acid was quantitated over the range of 5 2000 ng ml−1.
(B ) The gp120 signal associated with SIGN-R1+ SM or deeper in the IFC was quantitated over time following gp120 injection: 8 min-1 hr 14 min, upper; 1 hr 30 min–2 hr 30 min, middle; and 3 hr–32hr 32 min, bottom panel.
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Movement of unbleached fluorescent molecules into the ROI is quantitated over time and analyzed to determine the diffusion coefficient (D) of the fluorescent molecule [36].
HuR knock-down was determined to be >90% using Bio-Rad's Quantity One software (Bio-Rad) normalizing to β-tubulin, and HuR over-expression was quantitated in a similar manner.
The total amount of colocalization in each cell was quantitated by measuring and integrating the intensity values over all selected voxels in the Filipin UV channel.
The overall labeling of the anti-AtCNGC10 antibody over leaf and root cells roots was quantitated on a per cell basis.
Bound [3H]9-cis-RA was quantitated by liquid scintillation counting.
Aortic intimal thickening was quantitated in H&E-stained segments.
In addition, trypan blue (TB) was quantitated by light microscopy.
Serum OPG was quantitated using enzyme-linked immunosorbent assay.
Radioactivity was quantitated using a γ-counter.
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