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Aortic intimal thickening was quantitated in H&E-stained segments.
7-Hydroxycoumarin (7-HC) was quantitated in mouse plasma and urine.
The supernatant was quantitated in a mass spectrophotometer by the protocol described by Wright and Upadhyaya (1998).
Heteroplasmy was quantitated in individual cytoplasmic hybrid cells (cybrids), containing a large mtDNA deletion, and bulk cell samples.
The extent of derivatization was quantitated in terms of the mol of P-Ser/mol of peptide by 3H counting, and the location of the phosphoserine was defined by the N-terminal Edman degradation sequence analysis as being the fourth amino acid residue from the N terminus.
The plates were incubated for 24 hours and luciferase activity was quantitated in lysed cell supernatants in a luminometer.
PtdIns5P was quantitated in a neomycin affinity chromatography enriched phosphoinositide fraction using a radioenzymatic assay [6], [46].
For microarray analysis, the RNA was quantitated in a Beckman DU530 spectrophotometer and labeled cDNA was made using the Cyscribe labeling kit.
Real Time PCR assays for the three replicons were established, and the ploidy throughought the culture growth was quantitated in parallel with the chromosome (Fig. 4).
The % cell death was quantitated in random fields taken at ×250 magnification using the formula 'number of TUNEL+ nuclei/total number of nuclei x100' for each treatment group.
Virus was quantitated in plasma and CSF from 140 µl of plasma and CSF collected longitudinally as well as at the terminal time point.
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