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The protein content of each pooled sample was quantified again using a Bradford protein assay kit.
The purified cDNA was quantified again on a Nanodrop 1000 Spectrophotometer and diluted 40 times with nuclease-free water before being stored at −80°C.
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The relative contribution of each factor to these reactions is quantified, again, by a single parameter.
The time spent exploring each object and the total approach time were quantified again.
The purified PCR product pools were quantified again in 2% agarose gel prior to library construction.
c, d, e These data were quantified again in the present study to eliminate experimental bias.
The detailed expression patterns of these six genes were quantified again by qRT-PCR (second round of qRT-PCR).
Most of the data in ddY and FVB mice were quantified again in the present study to ensure comparability.
In order to confirm the accuracy of the quantitative results for the differentially expressed proteins that were identified, some proteins with an iTRAQ quantitative ratio were quantified again by Western blot analysis.
The samples then were quantified again and normalized in the same manner to a final concentration of 5 ng/μl, to ensure that the proper amount of genomic DNA would be used for the library preparation process.
However, in the present study, these were quantified again, together with the corresponding levels in C57BL/6J mice, in order to eliminate any experimental bias due to differences in experimental day and experimenter.
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