The hemofiltration circuit was primed with the same solution using a priming volume of 6.9 mL.
First-strand cDNA synthesis was primed with oligo dT), followed by RNA hydrolysis and adaptor-primed second-strand synthesis.
RNA was then reverse-transcribed using Superscript III that was primed with random hexamer primers (Invitrogen).
Each circuit was primed with human blood, and flow was set to 1 L/min.
The CPB circuit was primed with colloid solutions at variable volumes.
The feedback session was primed with stimulating questions and open-ended statements that were followed by listening and probing.
The start calibration routine ("LabVIEW algorithm" section) was modified so that at the end of the routine, the tubing was primed with the [18F]FDG solution.
Bone marrow was then drawn back gently (to prevent lysing of the cells) into the 30-ml syringe that was primed with 4 ml of ACD-A.
First, the 30-ml syringe was primed with 4 ml of anticoagulant citrate dextrose solution (ACD-A) (Arteriocyte Medical Systems, Hopkinton, MA).
The 30-ml syringe was primed with 4 ml of ACD-A before 26 ml of whole blood was obtained from each patient using standard phlebotomy procedures.
Human platelet-rich plasma (PRP) was primed with ADP, followed by stimulation with vehicle control, or HNP in the presence or absence of rRLN.
