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The training set was preliminarily checked for outliers.
Data distribution was preliminarily checked by the Kolmogorov-Smirnov test.
The linearity of the assay was preliminarily checked for each monoclonal antibody by submitting different amounts of untreated cell extracts to western blotting.
RNA quality was preliminarily checked by gel electrophoresis on a 1% agarose gel containing SYBR Safe™ DNA Gel stain 10,000× (Invitrogen™, Carlsbad, California).
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The DNA bundle and the intercalation results were preliminarily checked by scanning electron microscopy (SEM) and by fluorescence microscopy.
Violations of the latter assumption were preliminarily checked by looking at locus-specific deviations from HWE, and at mendelian consistency between parental and offspring genotypes.
Data were preliminarily checked for normality and equal variance using the Shapiro-Wilk and Levene tests.
All amplified PCR products were preliminarily checked by electrophoresis on 2.0% agarose gel and then observed under UV light.
After 1 week of cultivation, l-Glu-assimilating TcR-clones were randomly selected, and their chromosome structures were preliminarily checked by PCR using the primers P3: 5′-tgggacgaagagtacccgaacaaag-3′ and P4: 5′-cgctgacaagctggagcaggaaaaa-3′.
Each Nox candidate was preliminarily aligned with human Noxes to check whether the sequences conserved canonical regions required for O2- generation, such as the four heme-ligating histidines corresponding to His-101, His-115, His-209, His-222 of human Nox2 (GenBank™ No. NM_000388).
And the photocatalytic mechanism was preliminarily discussed.
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CEO of Professional Science Editing for Scientists @ prosciediting.com