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The separation method is described as follows: 0.1 min hold at 100% A, 14.9 min linear gradient to 100% B, 5 min hold at 100% B, 1 min linear gradient to 100% A, and 1 min hold at 100% A. A blank injection of water and 15 min chromatographic run was preformed between samples to eliminate possible carryover of analytes and to re-equilibrate the column.
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Heat shocks were preformed between 16 hours and 8 hours before puparium formation.
Loci-spanning probe melt analysis was preformed by continuous signal acquisition between 38°C – 65°C at default ramp rate of 0.6°C/s and 5 acquisitions per °C.
Statistical comparison between the results was preformed with regards to accuracy and precision using t-test and F-ratio at 95% confidence limit (Table 7).
Briefly, cross-linking between DNA and protein was preformed by adding formaldehyde (Merck) directly into the culture medium of mouse embryonic fibroblasts (MEF) derived from wt and Hpse-KO mice.
Statistical analyses were performed using SPSS version 17.0 (SPSS Inc., Chicago, IL, USA) and all data were expressed as mean ± S.E.M. Unpaired Student's t-test was preformed to investigate differences in EAAT3 activity between each drug concentration and control.
Headache was assessed according to ICHD-II (2004), Welch criteria were applied for the relationship between Mg and S. The Rankin score for S disability was preformed.
Was preformed 7 14 days prior to the Ramadan month.
Immunolocalisation was preformed as above.
MLST was preformed as described previously [9].
Nuclear transfer was preformed as described [53].
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