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Before the collection of volatiles, the fiber was preconditioned in the GC injection port at 250 °C for 20 min and then inserted into conical flask through the septum, afterwards exposed to the headspace for 40 min at 60 °C in a water bath.
Each SPME fibre was preconditioned in a hot injector at 250°C, a test chromatogram was recorded and the clean and activated fibre was then exposed into the sample headspace.
Desorption of volatiles from the fiber was placed in hot GC injector at 290°C, for 1 min. Prior every SPME sorption, the fiber was preconditioned in small needle heater (Gerstel, Mülheim an der Ruhr, Germany) at temperature 290°C, for 5 min. 20 ml headspace vials, teflon coated rubber septa and crimp caps were purchased from Gerstel (Mülheim an der Ruhr, Germany).
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Lead-free glass beads (0.5-0.75 mm) were purchased from Glen Mill Glen Milll's, Inc., Clifton, NJ, USA) and were preconditioned in-house.
It is preconditioned in such a way that, essentially, only the solution of the FE subsystems in each subdomain is required.
Endothelial cells are known to be preconditioned "in vitro".
Capillary morphogenesis by MS-1 was induced by the BMPCs, and the effect was enhanced when they were preconditioned in the presence of KP-1N cells.
The commercial SPME fibers were preconditioned in accordance with the manufacturer's recommendations.
MIP-coated SPME fibers were preconditioned in MeOH/water (90 10, v/v) for 15 min.
Before wear testing the gliding surfaces were preconditioned in the test solution until no increase of weight was measurable.
Neurons were preconditioned in minimal medium for 12 h, before addition of control or astrocyte-conditioned minimal medium supplemented with H2O2.
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