Sentence examples for was precipitated using the from inspiring English sources

From these fractions, L1 was precipitated using the anti-L1 monoclonal antibody 557.

According to the manufacturer's instructions, total RNA was precipitated using the RNeasy Protect Mini Kit (Qiagen, Crawley, UK).

The total Trizol volume was then increased to 200 μl and RNA was precipitated using the manufacturer's instructions.

RNA was precipitated using the nucleating agent linear acrylamide (Ambion, Austin, TX) as described by Gaillard and Strauss, 1990 [ 56].

Chromatin from undifferentiated and differentiated C3H10T1/2 cells was precipitated using the α-H3K9me3/S10ph and α-H3K9me3 antibodies and the DNA was hybridised to the microarray.

PLCγ2 was precipitated using the Q-20 PLCγ2 antibody (Santa Cruz Biotechnology, Santa Cruz, CA, USA) and captured using a 1 : 1 mixture of Protein A Sepharose (Zymed, South San Francisco, CA, USA) and Protein G Agarose (Invitrogen).

Pooled samples were precipitated using the ProteoExtract Protein Precipitation Kit (Calbiochem, Germany), according to the manufacturer's protocol.

Biotin-labeled proteins were precipitated using the streptavidin-agarose beads.

The protein concentration of each pooled CSF sample was determined using EZQ Protein Quantitation kit (Molecular Probes, Eugene, OR) and aliquots containing 25 µg of protein were precipitated using the ethanol/acetate method [18].

Nuclear extracts were precipitated using the 2D Clean-UP kit (GE Healthcare, Waukesha, WI) and resuspended in the appropriate 2D-DIGE lysis buffer (7 M urea, 2 M thiourea, 25 mM Tris-HCl pH 8.5, 4% CHAPS) [59].

Protein samples were precipitated using the two-dimensional Clean-Up Kit (GE Healthcare, Piscataway, NJ, USA) and resolubilized in T8 buffer.