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The suspending solution was precipitated for 12 h and the upper supernatant was collected and centrifuged.
Based upon the results of the hydroxyproline assay, the equivalent amount of each tissue digest containing 20 µg of total collagen was precipitated for blotting.
RNA was precipitated for 1 hour at -20°C.
The solution was precipitated for 6 h by adding three volumes of distilled water (v/v).
Total RNA was precipitated for 30 min at -20°C in 96% ethanol and 0.3 M sodium acetate.
By adding 1/10 Vol. NaAc pH 4.8 and two Vol. 96% ethanol, the DNA was precipitated for 1 h at -20°C.
Similar(43)
However, the amount of Sc that can be precipitated for hardening is rather limited because of its limited solubility.
After 3 min at room temperature and 15 min centrifugation at 13'000 g and at 4°C, 400 µl of aquous phase were precipitated for 10 min at room temperature in a fresh tube with 500 µl isopropanol.
Ligated fragments were precipitated for at least 2 hr at −30°C, purified on a 10% TBE-urea gel, eluted, and precipitated.
To this purpose, cells were exposed to10 nM rotenone, for 72 hours then protein were precipitated for polg and assayed with anti-HA antibody.
Protein and/or DNA complexes were precipitated for 1 h at 4 °C using 60 μl of 50% (vol/vol) protein G agarose/salmon sperm DNA slurry.
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