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For 2-D analysis of whole lymph, the lymph sample (10 mg protein) was pre-cleaned using the 2-D cleaning kit from Biorad.
The sample surface was pre-cleaned with argon plasma to remove surface contaminants.
The Si(100) substrate was pre-cleaned by standard method followed by HF etching to remove the native oxide layer.
The ITO-conducting glass substrate (a sheet resistance of 15 Ω/□) was pre-cleaned using acetone, ethanol, and DI water for 15 min each.
The FTO conducting glass substrate (with a sheet resistance of <15 Ω/□) was pre-cleaned using acetone, ethanol, and deionized (DI) water for 15 min each.
The electrically bistable device was fabricated as follows: the glass substrate coated with an indium-tin-oxide (ITO) anode was pre-cleaned and then the poly (3, 4-ethylenedioxy thiophene):poly- styrene-sulfonate) (poly- styrene-sulfonateted onto the substrate as a buffer layer and then annealed at 150 °C for 15 min.
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After the samples were pre-cleaned with protein G agarose, they were subjected to immunoprecipitation overnight with 2 μg of rabbit antibodies against G9a (Abcam) and H3K9me2 (Abcam), or with 2 μg of normal rabbit serum overnight at 4 °C.
Then the rod surface is pre-cleaned by sodium hydroxide solution.
The substrates are pre-cleaned using hot chromic acid, acetone and distilled water through systematic mechanism.
The glass had been pre-cleaned in a chromic acid solution for 6 h and after washing in deionized water was purified in an RF oxygen plasma.
The water samples were collected into 1 L polyethylene bottles which were pre-cleaned with concentrated hydrochloric acid and distilled water.
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