The mortgage itself was pooled with others and sold to investors — insurance companies, mutual funds and pension funds.
After the sedimentation of the residue, it was pooled with the previous one.
Polyester yarn with the tenacity of 31.77 cN/tex was pooled with 2.70 cN/tex copper wire in two different alignments (Figs. 1, 2).
This brought the total number of mentors included in the current study to 18. Demographic information about our mentors related to year in school, major, ethnicity, and gender is presented in Tables 1 and 2. Because only three graduate students participated in this study, their information was pooled with the UGs to protect the identities of participants.
Another 450 µl RIPA buffer was added to the sedimented lysate, centrifugation was repeated and 490 µl supernatant was pooled with the first one.
The supernatant was collected and the pellet was washed again in RSB+Mg2+ buffer, centrifuged, and the resulting supernatant was pooled with the original supernatant.
The remaining tissue was incubated for an additional 15 min under rotation, and the supernatant fraction produced after settling was pooled with the previous supernatant fraction.
Samples were sonicated for 5 min and supernatant was pooled with an additional peptide extraction round with 50% acetonitrile/1% formic acid for 20 min at RT.
Female status (laying vs. non-laying) was unrelated to the occurrence of extra-pair copulations (Fisher's exact test: Chi2 (1) = 0.3, P = 0.72), and so data from these females was pooled with those from females that laid eggs.
The filtrate was centrifuged at 1000 rpm for 5 min. The tissue pieces remaining in the tube were subjected to digest twice as before and the final supernatant was pooled with the first fraction after sieving.
Nuclear pellets were resuspended in RSBG40, and one-tenth volume of detergent [3.3% (wt/wt) sodium deoxycholate and 6.6% (vol/vol) Tween 40] was added with slow vortexing, followed by incubation on ice for 5 min. Nuclei were again pelleted and the supernatant was pooled with the previous cytoplasmic fraction.
