Larvae grown at the standard (lower) temperature were pooled between culture replicates at 5 d post-fertilization and those pooled larvae used in short-term treatments expected to affect gene expression.
Pooling risk Risk must be pooled between those who enjoy a long healthy life and those suffering chronic illness; between the young and the old.
Once all samples were collected, smaller subsamples were pooled between the two supplements to produce one pre- and one post-exercise sample to determine the effect of each modality on cortisol, independent of treatment.
Because the frogs spleens were pooled between tanks, we removed tank-level effects.
Samples were pooled between tanks but within experimental groups to remove tank-level effects.
When adjustments for covariates were needed, e.g. when outcomes could not be pooled between experiments, we instead used ANCOVA.
The following analyses were considered at country level, to assess whether the different language versions had similar psychometric properties, so that data could be pooled between countries.
Results were highly consistent between replicates within each experiment, and between experiments thus the data were pooled between the individual experiments and subjected to statistical analysis to yield the results shown.
Critical distance is particularly important if results of the MRS are intended to be pooled between different populations or regions, and less important if a study is planned as before-after-treatment-comparison in one country with only one language.
Critical distance is particularly important if results of the AMS are intended to be pooled between different populations or regions, and less important if a study is planned as before-after-treatment-comparison in one country with only one language.
Because we used a two-arm crossover design in this study, data were pooled between the two treatment phases after finding no carryover effect, and we report between-group differences at the end of study time points (week 8 and week 20) using general contrast of regression coefficients in the mixed-effects models.
