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Subsequently, litter was pooled according to species and origin of leaf litter, i.e., urban and periurban tree locations, yielding ten pools in total.
RNA from snap-frozen tumors (19 serous, four clear cell and 10 endometrioid tumors, respectively) was pooled according to histologic subtype.
Double stranded cDNA of sufficient quality was pooled according to genotype and treatment.
Urine from three males was pooled according to social status (DOM or SUB).
Since there was no effect of treatment or sampling time on metabolite concentrations, data was pooled according to gender.
In addition, data was pooled according to country in order to compare findings from the U.S, Australia and China.
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The samples were pooled according to Table 1 (five comparison groups with each pooled into three sub-groups).
After adding 10% protease inhibiting cocktail, 130 μL of whole blood from each individual sample were pooled according to Table 1 and Table 2.
Sera with anti-M2 reactivity were pooled according to affinity values for binding to M2, and high- and low-affinity pools were tested for their ability to prevent MV-induced encephalitis in a mouse model.
The two tanks per temperature and feeding were pooled, according to the procedure described in Underwood80, to check that variation among experimental units was zero, and that pooling was appropriate and did not change the conclusion of the analysis.
The data are pooled according to the comparative triptan.
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