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A marginal increase in discrimination between perfectly matched and 1 bp mismatched triplexes was observed upon extended incubation, indicating that the matched triplex complexes formed in the first 5 min, were stable throughout the 90 min incubation period which was periodically monitored.
Cell growth was periodically monitored by optical cell density measurement at 560 nm (OD560) using a Perkin Elmer UV/VIS Spectrophotometer (UV Vis Spectrometer: Lambda2S, Perkin/Elmer, Waltham, USA).
The flowthrough was periodically monitored spectroscopically: concentrations of free flavin were below the detection limit of 1 µM.
As 100% confluency alters the cells morphology, we performed our experiments in cells that were 70 80% confluent at the time of the infection with rgRSV at MOI (multiplicity of infection) ranging from 1 to 10. Virus multiplication was periodically monitored using a florescent microscope.
The reaction mixture was periodically monitored by UV visible spectroscopy to determine the extent of the conversion.
Her condition was periodically monitored and L-thyroxine therapy was no indicated, since her hypothyroidism remained subclinical.
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The mice were periodically monitored over an eighteen week period.
In addition, hypercalcemia can present at any time in a WBS patient's life; thus, although our patients did not have hypercalcemia during the assessment period, they are still at risk and should be periodically monitored for calcium disturbances [ 39, 40].
The status of this money is periodically monitored by the Nuclear Regulatory Commission to assure compliance with its regulations and adequacy to meet projected costs of decommissioning.
Smaller AAAs are periodically monitored with imaging.
Mice were periodically monitored following infection to record body weight, dehydration status, activity and behavior.
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