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The screening amongst formulations was performed preparing rod extrudates and using the extrusion profiles to assess their suitability for extrusion and to anticipate quality of the spheronized extrudates.
The screening amongst formulations (13 different blends) was performed preparing pellets and evaluating extrusion profiles and quality of the spheronised extrudates.
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Evaluation was performed by preparing a corpus of experimental paragraphs from the chemical literature and conducting an inter-annotator study.
Dilution integrity test was performed by preparing samples at a concentration approximately two times the concentration of 90 % ULOQ.
Measurement was performed by preparing three-dimensional constructed models and thereafter taking a sagittal section at the region with maximum IC diameter.
Bile solubility testing was performed by preparing a bacterial suspension in 1 mL 0.85% NaCl (McFarland standard 1.0) adding four drops of 10% sodium deoxycholate.
Excavation was performed after preparing occlusal cavities to access the carious lesions.
This was performed by preparing two series of serial two-fold dilutions of each conjugate (20 μ M 110 n M) in RPMI-1640 (containing 10% FCS).
Mueller-Hinton agar was purchase from Sigma-Aldrich; antimicrobial growth was performed locally prepared at laminar flow cabinet.
Last, just a chemical surface cleaning was performed to prepare the steel adherend surface, i.e. no abrasive method was used, just acetone surface cleaning.
Pore-widening process in 10% phosphoric acid (10 min to 1 h) was performed to prepare AAO with equal inter-pore distances but different pore diameters.
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