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Two-tailed paired Student's t-test was performed, data represent means ± s.e.m. of n = 4 independent experiments.
c, SDS PAGE with 2 μg protein loaded for each mtIF2 variant to show that protein concentrations have been estimated correctly for all variants before in vitro translation was performed (data for mtIF2(H678A) not shown).
Quality data extraction was performed, data sets were analyzed for significant differences and patterns predictive of TB+/−.
As internal normalization control, a PCR using methylation insensitive primers (for gnDNA: forward, 5'-CTCCAACTCAGGGCCTACAC; reverse, 5'-CCAGGCTTTTGTGGCCTAT in combination with SYBR green; for pOctTK plasmid: forward, 5'-ACTGCATCTCCCTTTCCTTGT; reverse, 5'-GCCCCCTGCAAGTCTTTT in combination with Roche UPL probe #137) was performed (data not shown).
To establish SNVs, a two-step blast search was performed (Data S4).
A literature search was performed; data were also collected from the European Medicines Agency website.
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A thematic data analysis was performed and data were analyzed for meaning.
A calibration was performed with data from the literature.
DESIGN: Predictive modeling was performed using data extracted from a randomized controlled trial.
Statistical analysis was performed on data on intracellular IDO staining.
Real-time RT-PCR was performed and data were analyzed as reported previously [61].
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