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Adenovirus-mediated gene transfer was performed by adding a minimal volume of medium containing 2% FBS and adenovirus.
More importantly, the reversibility of the recognition process of HL was performed by adding a Al3+ bonding agent Na2EDTA.
Lentiviral transduction was performed by adding the virus supernatant to primary cells maintained in ultra-low attachment plates (Corning) to a final volume of 500 μL.
Ca2+ influx analysis was performed by adding 6.25 μM ionomycin (Wako) or ionomycin and 1 μM ruthenium red (Sigma) to cultured cells.
The surface-capping process was performed by adding 0.7 ml of 1-vinylimidazole into the solution.
Quenching of the reaction was performed by adding methanol and chlorotrimethylsilane basified with TMAH pentahydrate.
Terrain classification was performed by adding surface texture data to LN slope and local convexity.
The selection was performed by adding puromycin (Sigma Aldrich, Saint Louis, Missouri, USA) to cell culture medium every 48 hours.
The recovery was performed by adding known amount of individual standards into a certain amount of sample HN.
Recapping of the nanocrystals was performed by adding the dry nanocrystals to dodecylamine heated at 200 °C under nitrogen atmosphere.
The mixing process was performed by adding water (480 ml/kg fresh matter) to SM, PW and U, followed by M or BB.
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