Exact(1)
The IPG well of the gel was overlay with agarose sealing solution (0.5% w/v prepared with 50 mL of Lammeli running buffer and traces of bromophenol blue).
Similar(59)
The outcome of FESEM information (Fig. 8) was overlayed with earlier reports [23, 24].
When the crazing condition, which increased linearly with AN content, was overlayed with the interfacial toughness, the intersections correctly predicted the AN content for transitions in failure mode.
After 1 hour, top agar was overlayed with 1 ml fresh media.
Subsequently, the gel was removed and the agarose was overlayed with 20 ml of double-strength nutrient agarose (6% TSB, 1% Bacto-agar, 45°C), and plates were incubated overnight at 37°C.
Infected cell lysate in 71.5% sucrose solution was overlayed with 55% and 10% sucrose solutions, and after ultracentrifugation, 13 fractions were collected and the presence of NP in the fractions was determined by Western blot analysis using anti-SeV polyclonal serum.
The ensemble labelling was overlayed with the SPT data and the frequency of colocalizing trajectories calculated.
The LA-ICP-MS contour plot was overlayed with a sample image taken before the analysis using an installed microscopic camera.
MRS hard agar (1% w/v) was overlayed with MRS soft agar (0.75% w/v) preseeded with approximately one million cells of indicator Enterococcus faecalis.
This was overlayed with a 4 ml volume of 35% (w/v) sucrose in HB, followed by a 4 ml volume of 5% (w/v) sucrose in HB.
The suspension was overlayed with 15 ml of 20 mM Tris-acetate (pH 7.5) containing 0.25 M sorbitol, 1 mM EGTA, 2 mM DTT and 2 mM MgCl2.
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