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In agreement with the pulse experiments shown in Fig. 2D, newly synthesized cytoplasmic 20S pre-rRNA was only efficiently converted into 18S rRNA when rpS5, however not rpS5-ΔC was expressed.
When internalization of virus was assessed by immunofluorescence staining using an anti-NP antibody, infection of cells was only efficiently prohibited if virus was adsorbed in the presence of iota-carrageenan but not control polymer (Figure 5C) or if iota-carrageenan or control polymers were added post-adsorption (Figure 5D).
We observed a very high background of recombinant Orc1-1 protein bound to both DNA substrates and even to beads alone (data not shown), but MCM was only efficiently recruited to the origin-containing DNA substrate.
While the unprocessed HA-tagged PfRh1-120 protein was readily detected using all three detergents, the cleaved stub, which ran with an apparent molecular weight of ∼16 kDa, was only efficiently detected when TNET was used to extract proteins (Fig. 6E).
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Microinjected transcripts were only efficiently spliced if they were capped, and splicing of the 5′ proximal intron required 7mG [ 75].
This effect is well illustrated by exon 37 processing of the NF1 gene, which is only efficiently recognized in minigenes with at least exons 34 38 [Buratti et al., 2006; Baralle et al., 2006].
Therefore, it is speculated that expression of the bukII is only efficiently activated when fermenting some non-preferable sugars (such as d-xylose and l-arabinose) because, compared to d-glucose metabolism, a higher ATP demand is required then [ 74].
PLC independence may play a role in contributing protection from arthritis by a number of potential mechanisms such as conferring an inability to bind an athritogenic peptide that is only efficiently bound in the context of the PLC.
Our studies on the Raji cured cells have led us to suggest that proteases present within these tumours are responsible for the enhanced reovirus infection in vivo, since these cells are only efficiently infected in vitro when reovirus is first proteolytically processed into ISVP before infection.
However, the Half DVD-Ig molecule was only able to efficiently trigger TCR signaling in the presence of tumor cell antigen which likely functioned as an anchor to help stabilize CD3 binding to the T cells and promote conditional TCR crosslinking (Fig. 5).
Zero-order could be applied only at low temperatures or until the concentration of NaBH4 remained high in the solution; first-order could be only applied efficiently at 60 °C.
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