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Similarly, the PrgI sequence was only aligned to other T3SS needle proteins.
Each read was only aligned to one site in the reference sequence; if reads were aligned to different references sequences then the reads were discarded.
a) n/a: Not available; b) Calculated according to: #pseudogenes/(#pseudogenes + total proteins)*100; c) The genome of ATCC BAA-102T was only aligned to that of CJ18 but not completely assembled.
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Genes (i.e. tags that matched a transcript) and tags that were only aligned to the genome were analyzed separately.
In the KEGG pathway, TDC belongs to indole alkaloid biosynthesis [PATHWAY: ko00901], which is only aligned to STR, not aligned to the TDC.
However, there are deficiencies associated with the databases as the Monoterpenoid biosynthesis [PATHWAY: ko00902] in KO is only aligned to myrcene/ocimene synthase (EC 4.2.3.15).
Importantly, however, it was found that growing mineral crystals are only aligned when the assembly of the protein and the growth of the crystals occur simultaneously.
In traditional methods, a query is only aligned with the sub-sequences of the genome; these sub-sequences are generally less than the maximum length [ 13, 18].
Using these parameters, reads were only aligned when their relative orientations are correct and their inferred distance along the assembly (the aligned span size) was consistent with the estimated library insert size.
The domain compositions of the short coronin, the class-3, and the class-4 coronins are different and regions outside the N-terminal coronin domain were only aligned within these groups.
In the pair-wise comparisons between Novoalign and any of the other three aligners for S2 data set, the number of reads assigned to the first class increases almost 3-fold, (1.2 million vs. 3.7 million), while the number of reads that are only aligned by the opponents of Novoalign become markedly less (see class 2 of Table 8), compared to non-trimmed alignments (see class 2 of Table 6).
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