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We also performed both no-template controls and reactions with RNA that was not reverse transcribed (to confirm the absence of genomic DNA contamination).
No-template controls and reactions with RNA that was not reverse transcribed were performed in order to confirm the absence of genomic DNA contamination.
In all cases, appropriate controls were performed to demonstrate that no bands were detected when the OE RNA was not reverse transcribed.
In addition to removing DNA from the RNA template by enzyme digestion, false expression signal from contaminating DNA was assessed by including RNA that was not reverse transcribed.
An aliquot of RNA that was not reverse transcribed served as a control PCR template for DNA contamination.
To control for genomic DNA contamination, PCR amplification was performed on template RNA that was not reverse transcribed.
Similar(51)
It was not reversed.
It was not reversed immediately.
The trend was not reversed until the middle of the 15th century.
Despite Fowler telling Ashby he had not been fouled by Arsenal goalkeeper David Seaman, the decision was not reversed.
However, this process was not reversed by caspase inhibitors.
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