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Since different sites had microstimulation sessions of different lengths, this area was normalised across sites by division by the total number of trials in the microstimulation session at each site.
Xenophobia was normalised across the continent.
Raw expression data collected from an Affymetrix HP GeneArrayScanner was normalised across all data sets using the RMA algorithm.
The data was normalised across library sizes, between samples using the trimmed mean of M-values (TMM) normalization method [ 70].
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Since the internal standard is normalised across all the gels, separation of inter-gel experimental variation from biological variation is possible [ 11, 102].
Each array was normalised individually, followed by normalisation across all arrays to allow comparison.
First, the summed fluorescence across pixels within the defined area was normalised and then plotted to give a measure of integrated density of the fluorescence.
MMP expression was normalised to GAPDH.
Data was normalised to DNA content.
Expression was normalised to GAPDH Cell Signaling Technologyy).
Intensity was normalised to GAPDH.
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