Sentence examples for was next added from inspiring English sources

Western blot loading buffer was next added to the beads and incubated at 95°C for 10 min to denature protein.

The tane koji (10 g) was next added to the steamed rice and its rice-koji was prepared by incubation at 30 °C for 40 h.

C1 phage was next added and incubated for 1 hour, and bound C1 phages were measured with anti-M13.

Pre-warmed DPBS was next added and the surface of the well washed by moving the rack between 0° and 30° and back five times.

The antibody treated medium was next added to human middle ear epithelial cells transfected with the β-defesnisn 2 promoter construct.

An equal volume of chloroform methanol (v/v, 2 1) with the culture was next added to extract the lipids [ 36].

Indicated amounts (Fig. 4D) of antibody were next added.

Diisopropyl ethylamine (13.0 ml, 75.19 mmol) and a catalytic amount of DMAP (57 mg) were next added to the flask.

The 15 non-malignant brain sample data sets were next added to the entire dataset, and z-normalization and hierarchical clustering was repeated.

The Kinase-Glo Reagent is next added to terminate the PKA reaction and detect the remaining ATP via a luciferase reaction.

These fragments were next added to the Repeat library and each whole genome sequence was masked with the other three strains 30 bp fragments using WUBlast and RepeatMasker programs as described previously.