Sentence examples similar to was necessary previously from inspiring English sources

This was necessary since the previously described liquid overlay method used RPMI culture medium for basic cell line culture, this was replaced by DMEM/F12 to better accommodate primary tumor cells.

This is likely, given than that migration from more southern latitudes was necessary to colonize these previously glaciated areas.

This step was necessary since the microarrays previously used for inter-species CGH in the L. acidophilus group [ 26] were amplicon-based and not oligonucleotide-based.

It was necessary to align many previously known fungal orthologs of Nbs1 and then use this alignment to analyze the C. cinereus genome.

Previously it was necessary to use NMODL for reaction-diffusion.

The new representation allows us to process the complete set of examples, while previously it was necessary to sample the negative examples.

Previously, it was necessary to use Google Cloud SDK via the terminal.

Our GBS-based map does not share any markers with the two previous maps, so it was necessary to identify positions of previously generated markers in Astyanax to enable comparison between previous mapping efforts and those described here.

In order to resolve individual etch pits associated with the indentations, it was necessary to reduce the etching time previously defined to approximately one second.

Since our findings differ substantially from those reported previously [22], it was necessary to ensure that the CACNA1F gene mutation in the Cacna1fnob2 mouse line that we were testing was the same as that originally described.

As previously discussed, it was necessary to evaluate the soluble fraction of pneumolysin, which is the active fraction, expressed in all 24 different operational conditions, to define the most appropriate expression condition and then compare it with the condition for the best cell growth to determine whether the soluble rPly expression was correlated to cell growth.