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The strain was maintained frozen at −80 °C with 20% (w/w) glycerol added.
The strain was last stored in 1988 and was maintained frozen until shipping to Oxford in 2008.
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Stock cultures in 25% glycerol were maintained frozen at −80°C.
This valuable material can be maintained frozen for years and further used to perform independent experiments.
All C. acetobutylicum and E. coli strains were maintained frozen in 15% glycerol at −80°C.
Collectively, these data indicate that viable BM cells can be maintained frozen, and once thawed, they can readily be used to generate BMDM.
Aliquots were maintained frozen at –70 °C.
The bacterial cultures were maintained frozen at –80°C in cryovials.
Finally, the tissues were maintained frozen in our tissue bank.
The isolates were maintained frozen at -20°C in Trypticase soy broth (Difco Laboratories, Michigan, USA) containing 10% glycerol until testing.
The fresh samples were analyzed on the day of blood draw, whereas the cryopreserved samples were maintained frozen for at least 1 week prior to analysis.
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