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Then, E. coli BL21L was made competent again and co-transformed with the pET-SIDF and pET-SIDFG plasmids, obtaining the strains E. coli BL21LF and E. coli BL21LG, respectively (Table 1).
E. coli DH5α strain was made competent by CaCl2 method and the GFP plasmid was transformed by heat shock method.
The W(R) yeast was made competent using the S.c.
The transformant harboring pACCAR25Δ crtE was made competent in accordance with the method of Inoue et al. [ 55] and then was transformed with pET- EgcrtE.
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These strains were made competent using induction of pLK as described above.
Cells were made competent and transformed with 1-2 µg of the previously described [8] selectable (URA3) high copy GAL::BRCA1 yeast yeast BRCA1 expression plasmid (BRCA1 plasmid).
All strains and plasmids are listed in Table 2. B. subtilis cells were made competent for transformation with DNA either by the method of Kunst and Rapoport [41], or by the method of Anagnostopoulos and Spizizen [42] as modified by Jenkinson [43].
Salmonella cells were made competent by standard methodology.
All pneumococci strains were made competent as follows.
Proliferative cells are made competent for DNA replication and mitosis at the G1/S and G2/M boundaries.
E. coli strains were made competent and transformed using standard techniques [ 33].
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