Briefly, samples were fortified with stable isotope analogs of the target analytes, and glucuronide or sulfate-bound metabolites were liberated using an enzyme hydrolysis.
Adherent cells were liberated using GIBCO™ cell dissociation buffer enzyme free (Invitrogen) and resuspended in Kreb's Ringer phosphate (KRP) buffer (PBS containing 1 mM CaCl2, 1.5 mM MgCl2, 5.5 mM glucose, pH 7.4).
For example, the hydrolysis by endo-1,4-β-mannanase from Aspergillus niger BK01 showed that mannobiose was the main product and mannose was not liberated using locust bean gum as substrate [ 5].
The obvious benefit: rather than being obligated to sit in front of the defense, Jones was liberated to use one of his prime virtues, his ability to cover ground, to have a greater influence.
The camp was liberated in 1945 but used by the Soviets to house political prisoners until 1950.
When they began retreating, after the Allies landed and before the region was liberated, mid-September 1944, we were using these small bombs to blow up German trucks on the highway.
Biotin was liberated from pure PfBCCP-79 using acid hydrolysis, and was quantified in a bioassay based on the growth of a biotin auxotroph E. coli strain.
Sucrase activity was determined by measuring the glucose liberated using glucose kits (Merck, Germany, Cat # 1.16720.0001), while levan formed was measured using the following procedure: 500 μl of the sample was added to 750 μl ethanol (to precipitate levan), and then the mixture was centrifuged.
Over 3 decades ago a correlation was described between the neuraminidase activity in the middle and distal small intestine and the milk Sia content that led the authors to suggest that milk Sia is liberated and used by the suckling newborn [18].
