Exact(5)
A two-stage multivariate optimization approach was developed by means of a Plackett Burman design for screening and selecting the significant variables involved in the USADLLME procedure, which was later optimized by means of a circumscribed central composite design.
A two-stage multivariate optimization approach has been developed employing a Plackett Burman design for screening and selecting the significant factors involved in the USA-DLLME procedure, which was later optimized by means of a circumscribed central composite design.
A two stage multivariate optimization approach has been developed employing a Plackett Burman design for screening and selection of the significant factor involved in the in situ IL-DLLME procedure, which was later optimized by means of a circumscribed central composite design.
The Dvorak layout was later optimized for the French language, which gave birth to BÉPO — a layout that still has fans to this day.
PRC coded the model and simulations in MATLAB, which was later optimized by PRC, PHC, and YH.
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A two-stage multivariate optimization approach was developed by means of a Plackett Burman design for screening and selecting the significant variables involved in the SDME procedure, which were later optimized by means of a circumscribed central composite design.
The groups of compounds were later optimized by k-means algorithm, minimizing the Euclidean distance to the group centers.
Conditions under which crystals were obtained were later optimized in 24-well Limbro plates (Hampton Research, Aliso Viejo, CA) using 2 μL hanging drop volumes.
Initially, the reaction conditions followed [ 42] (i.e. 90 s at 94°C, 5 × (30 s at 94°C, 30 s at 54°C and 1 s at 72°C), 38 × (15 s at 94°C, 30 s at 54°C and 5 s at 72°C) and 10 min at 72°C) but were later optimized to exclude the extension phase in order to minimize nonspecific amplification of longer fragments.
This model was later further optimized by conditioning Myc expression using the Cre- loxP (Langenau et al., 2005) and heat shock promoter (Feng et al., 2007) systems, and, more recently, extended to a tamoxifen-inducible version of Myc under the control of the rag2 promoter (Gutierrez et al., 2011).
The dextrose concentration of the dialysate was later increased to 2.5% to optimize ultrafiltration.
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