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The PCR product was later inserted into the plasmid vector (pCR-Blunt II-TOPO; Invitrogen) and used to transform competent E. coli cells (Invitrogen).
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In two of these patients, chest tubes were later inserted for asymptomatic increase in size on a 6-h film.
These enzymes embed surface proteins in the cell wall through a transpeptidation reaction that involves recognizing a penta-peptide "sorting signal" in a target protein, cleaving it, and covalently attaching it to a second substrate that is later inserted into the cell wall.
This would generate the pattern that we observe if this IntI-associated copy were later inserted into the same integron.
Adapted 0.5 ml micro-centrifugation tubes (cap and base removed) were later inserted into the punched holes to act as cell injection and collection reservoirs.
Three arrays, consisting of 150- μm wide and 1.5-mm long optrodes with 45° tips, were tested in 2% agarose; the same arrays were later inserted post mortem in cat brain (dura removed) and sciatic nerve.
The trajectories for the cortical screws are slightly medial or lateral to the central line of the plate to avoid entering the proximal radioulnar joint and to leave room for an axial cancellous screw, which is later inserted through the most proximal hole along the shaft of the ulna.
It was later re-inserted successfully.
The obsessive toy collector known as Al McWhiggin, who had appeared in a draft of Toy Story but was later expunged, was inserted into the film.
The map was later refined using microelectrodes inserted in the depths of cortex to stimulate a small cluster of neurons using trains of electrical pulses by a method that came to be known as intracortical microstimulation (ICMS; e.g. Strick & Preston, 1978; Preuss et al., 1996).
One of these was in a patient with an ununited fracture of the navicular where a peg graft of cancellous bone was inserted, which was later absorbed.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com