Contrast is largely concentration and intensity independent but sensitive to the solvent environment of the fluorophore, for example pH (26), Ca2+ concentration (27), protein-bound NADH fraction (28), refractive index (29), or viscosity (30).
TR-FRET assays detected a largely linear signal for the α-syn protein, but the variation was higher than AlphaLISA signals for every concentration tested, and the linearity was largely abolished at concentrations below 25 ng/mL (Fig. S3C and S3D).
The magnitude of CO response of the EC(2.0Au/In2O3(400air)) sensor in wet synthetic air showed a good linear relationship with CO concentration, but the CO response was largely affected by the concentration of O2.
Moreover, the concentration was largely underestimated for the duration of the period.
Even in small dendrites, the rise in calcium-bound indicator concentration was largely independent of distance to the membrane.
This concentration was largely compatible with those adopted in the functional in vitro tests.
Again, the absorption peak at 415 nm of the RtelN iron-sulfur cluster was largely abolished as the concentration of nitric oxide was increased).
Their activities were effectively inhibited by 1 μM M13 in the absence of added CaM; however, in the presence of 1 μM exogenous CaM, this (IC50) concentration was largely ineffective.
Luminal application of the same TDCA concentration was largely ineffective, whereas 1 mmol/L TDCA stimulated GLP-1 secretion (1 mmol/L TDCA was not tolerated when applied via the vasculature, Figure 4C).
However, the peak obtained with these regimens was largely below the desired concentration of 64 μg/ml, suggesting that higher doses of amikacin should be administered to achieve optimal peak levels.
