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The pncrA fragment was labeled using infrared dye-labeled M13 oligos and purified [29].
The pncrA fragment was labeled using infrared dye-labeled M13 oligos and purified as described previously [29].
1 mg of DNA was labeled using either 5' Cy3 or Cy5-labeled Random Nonamers (TriLink Biotechnologies).
Total RNA (20 μg) was labeled using a Fluorescent Direct Label Kit (Agilent Technologies) and simultaneously reverse transcribed into cDNA.
Both the purely spatial and space-time models were constructed using only event (i.e. count) data, but each event was labeled using its quartile ranking.
F-Actin was labeled using Phaloidin-633 Alexa Fluor (Invitrogen).
As a practical alternative, a subset of axons was labeled using MFas II (C3) immunoreactivity.
Total RNA was labeled using the miRCURY LNA microRNA Array power labeling kit (Exiqon Inc., Denmark).
After inactivating the enzymes 20 minutes at 65°C, the DNA was labeled using the Bioprime arrayCGH Labeling kit (Invitrogen).
DNA oligonucleotide was labeled using a Biotin 3' end DNA Labeling Kit (cat #: 89818, Pierce Biotechnology Inc., Rockford, IL).
For each array 1.5 µg total RNA was labeled using the mirVANA™ miRNA labeling kit (Ambion) following the manufacturer's instructions.
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