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DOP-PCR-amplified chromosome-specific DNA was labeled during the secondary PCR by incorporating biotin-16-dUTP (Roche, Switzerland) or Cy3-dUTP (GE Healthcare, UK) [ 48].
The mRNA samples corresponding to treatment (infested) and control (non-infested) was labeled during the cDNA synthesis with Cy3- or Cy5- labeled dUTP and with one technical replicate labeled by reversed dye compared to the first hybridization.
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Due to the rapid first-pass extraction of 99mTc-HMPAO [ 29], brain images reflect the average activity that was labelled during the third and fourth blocks.
For viability and cell cycle analysis, cells were labeled during the last hour of culture with the DNA dye Hoechst 33342 (SIGMA) (5 µg/ml, 60 minutes in incubator at 37°C with 5% CO2).
RNA samples were labeled during first-strand cDNA synthesis.
In principle, by carefully choosing Cre and loxP transgenes, a small set of hematopoietic cells can be labeled during a specific temporal window.
To perform this experiment, cells were labeled during a 60 min pulse of BrU followed by a chase with excess uridine for up to 180 min.
We found that epithelial cells with these characteristics are labeled during mammary ductal development, and that after a long chase period they continue to retain both the original DNA label and expression of PR and ER-α.
From the responses provided by the participants several sub-themes were labelled during the coding of the transcripts.
Two portions of N315 cDNA were labelled during reverse-transcription, with either Cy-3 or Cy-5 and competitively hybridized on StaphChip.
The samples were labelled during 30 min in a volume of 100 µl of Annexin-V buffer 1× and diluted to 600 µl before hs-FCM analysis.
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