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Total cellular RNA was isolated utilizing the NucleoSpin RNA II kit (Macherey-Nagel).
Subsequently, total RNA was isolated utilizing the RNeasy Mini Kit (Qiagen, Hilden, Germany) according to the manufacturer's recommendations.
Briefly, the cells untreated or treated with CuE for 4 h were harvested, and total RNA was isolated utilizing an RNasey kit (Qiagen, Hilden, Germany) as described by the manufacturer.
Poly(A) mRNA was isolated utilizing oligo dT) beads, fragmented and random hexamer-primers were used to synthesize single-strand cDNA, followed by a second round of cDNA synthesis.
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Primary human NK cells were isolated utilizing RosetteSep Human NK cell Enrichment cocktail from Stemcell Technologies.
Single cell-derived clones were isolated utilizing fluorescence-activated cell sorting from genetically engineered 231-BrM2 (WT10, MUT3) and CN34-BrM2 (WT6) cells.
DNA and RNA form BCLs were isolated utilizing RNeasy Mini Kit (QIAGEN) and used for QPCR and RTQPCR in a LightCycler FastStart DNA Masterplus SYBR Green I (Roche, Mannheim, Germany) (supplementary method).
In this study, pMSCs were successfully isolated utilizing the property of adherence to plastic, and following culture, the early cell population showed spindle, flattened, star and round morphologies.
Genomic DNA of B. gibsoni was isolated and utilized as a positive control from infected blood sample showing parasitemia in blood smear examination.
Genomic DNA was isolated and purified utilizing the Qiagen DNeasy plant maxi kit.
Six novel meroterpenoids cochlearoids F K (1 6) were isolated by utilizing phytochemical approaches.
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