Sentence examples for was inserted to modify from inspiring English sources

The thin TiOC layer with smaller surface energy and lattice constant than MoC was inserted to modify the magnetic properties and microstructure of FePt films.

The modified ccdB gene was inserted to a BstXI gap of pSHIN-G.

A 1.3-kb fragment of genomic DNA bearing exons 3 and 4 of the BHD gene was inserted into the modified pDONR vector pENTR3C-loxPMCS-loxP-FRT-neo-FRT between the SalI and NotI sites to generate a BHD-exon3-4-pENTR3C entry clone.

In construct ZEGFR:1907, affibody was inserted into the modified pET21a vector using HindIII and XhoI sites.

This was inserted into a modified pEGFP-1 expression vector (Clontech, Palo Alto, CA, USA) in which KpnI/ XbaI sites were created by removing the EGFP gene.

The gene for d4Venus was inserted into the modified pEGFP-N1 plasmid, which lacks the CMV promoter and contains the SV40 poly-A region, in place of EGFP.

An EcoRI-KpnI fragment of the RolC promoter [ 32] was inserted in the modified pGPTV-HPT and a SacI restriction site was removed by treating with the T4 DNA polymerase [ 33].

The 5′ arm was inserted into the modified pBluescript first, followed by the 3′ arm and then an antibiotic resistance cassette (either neomycin, puromycin, blasticidin or histidinol) was introduced into the BamHI site.

The TetR coding sequence was inserted into a modified pCAG-IRES-Puro mammalian expression plasmid, between the coding sequence for an N-terminal FLAG STREPx2 (FS2) tag and a 3′ ligation-independent cloning (LIC) site.

The 3xNLS-TetR fragment was inserted into a modified pCAG-IRES-Puro mammalian expression plasmid, between the coding sequence for an N-terminal FLAG STREPx2 (FS2) tag and a 3′ ligation-independent cloning (LIC) site.

The fragment was inserted into a modified pET-15 vector (Novagen), enabling cytoplasmic expression of the SitA protein with an N-terminal 6-His tag followed by a cleavage site for the TEV (tobacco etch virus) protease.