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The bacterial suspension was inoculated over entire surface of Muller Hinton Agar (MHA) plates uniformly using a sterile spreader.
The spore preparation (0.1 mLs) was inoculated over a toilet seat surface area that was equivalent to the diameter of a Rodac plate and allowed to dry overnight.
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Some 20,000 children will be inoculated over the next 3 years in Asia and Latin America with Takeda's TAK-003 dengue vaccine.
Mueller-Hinton agar plates supplemented with sheep blood were inoculated over the entire surface of the agar with drug-resistant bacteria by spreading with sterile cotton swabs.
Briefly, a 50 mL culture of transformed Y187 yeast cells was inoculated from an over-day 5 mL culture in minimal media lacking Trp.
A new MTP was filled with 150 μl 2xYT-GA medium and each well was inoculated with 10 μl over night culture from the respective well of the master MTP and incubated for exactly 2 h at 37 °C and 300 rpm.
Overall, 5 ml over-night culture was inoculated to 500 ml LB medium (1/100) with kanamycin and cells were further grown at 30 °C to OD600∼1.
Subsequently, 50 ml of prewarmed KB medium was inoculated with 1 ml from the over-night culture and incubated for another 5 6 h at 25°C.
Etest was performed according to the manufacturer's instructions Briefly, each 150-mm petri plate containing 60 ml of medium was inoculated by streaking the swab over the entire surface of the medium.
Further, a layer of Mueller Hinton agar medium (for bacteria) or Saboraud dextrose agar (for yeast) containing 1.5 × 106 cells/mL of each microorganism suspension separately was inoculated and sprayed with fresh culture over a developed TLC plate and incubated for 24 h at 35°C (for bacteria) and 48 h at 28°C (for yeast).
After thawing and mixing the cryoculture, another MTP was inoculated and a second preculture was conducted over night.
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