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A reactant was constructed for which the QM/MM minimization procedure was initiated by using RESD [36] restraints for bonded atoms P (3DR7) and O3′ (CYT6) so that they remained separated at 1.6 Å and for OH− (water 1863) and P (3DR7) which remained 3.5 Å apart.
The PCR was initiated by using the telomerase mediated elongation products (i.e. the telomeric DNA repeats) as template.
The RMD simulation was initiated by using unambiguous NMR distant constraints on the peptide without the presence of the disulfide bond.
Subsequently, testing for other respiratory viruses, including respiratory syncytial virus, parainfluenza viruses 1 3, human metapneumovirus, adenoviruses, and rhinoviruses, was initiated by using the respiratory samples.
First-strand RT was initiated by using Superscript II (Invitrogen) with a random octamer linked to a specific primer sequence (5′-GTT TCC CAG TAG GTC TCN NNN NNN N-3′) (14 ).
The characterization of the deletion was initiated by using five sets of primers for PCR (1 5; Supplementary Table 2) in patient II.6, his mother (I.2) and a control individual.
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We investigated the effect of the setting where the outbreak is initiated by using 2 scenarios.
The studies were initiated by using ten-fold serially diluted methanolic extracts of each plant followed by further optimisation in order to achieve a specific cytotoxic concentration.
After attaining an activating clotting time (ACT) greater than 480 s, CPB is initiated by using an occlusive roller pump (Jostra, Germany) and a membrane oxygenator (Affinity7000, American) followed by moderate hypothermia (28°C) and crystalloid cardioplegic cardiac arrest.
By contrast, in those patients who exhibit A1C levels above 7.5%, it has been demonstrated that the basal hyperglycemia becomes predominant, and therefore any treatment to improve diabetes control should be initiated by using medications that mainly act on fasting and interprandial glucose.
Spiral wave reentry was initiated by use of S1S2 stimulation, with the delay timed to occur within the vulnerable period (VP).
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