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The FE modeling strategy was initially verified and then used to solve for the maximum stress concentration factor at the countersunk hole.
The specificity of the antiserum was initially verified by ELISA and Western blotting in comparison with antiserum provided by Richard Bram (Mayo Clinic, Rochester, Minnesota).
RNA quantity was initially verified using a Qubit fluorometer (Life Technologies).
Each probe was initially verified by FISH mapping onto metaphase spreads generated from lymphocytes from a normal, healthy individual.
The specificity of PaD97-D6, PaD213-A52, Pand13-A5, PaD236-H2 and PaD236-H2 was initially verified on different forms of Aβ42, i.e. monomers, small, medium and large oligomers and mature fibrils by ELISA.
Aneuploidy of the respective chromosomes in each of the strain panels was initially verified via aCGH and subsequently via genomic sequencing, with periodic confirmation by qPCR to ensure aneuploidy maintenance.
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The model has been initially verified by coupling the atomistic-continuum domains in a representative volume element of the nanocomposite.
The numerical procedure is initially verified by the analytical solutions, and then the calculated directivity gain patterns are demonstrated for the modeled horn antenna configurations.
In the study, the DSC method is initially verified by comparison with classical laminated plate theory for simple supported, six-layered, specially-orthotropic composite plates for modal and response analysis throughout a broad frequency range.
All PCR products were initially verified by sequencing.
The sizes of the PCR products were initially verified by agarose gel electrophoresis and subsequently checked by melting point analysis after each reaction.
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