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Diabetes was induced with a single intraperitoneal dose of STZ (65 mg/kg body weight).
Expression of UNC-45 full-length and UNC-45 point mutants was induced with a final concentration of 100 µM IPTG for 12 h at 18 °C.
Stress was induced with a speech task, followed by a bogus taste task paradigm designed to assess unhealthy food consumption.
Experimental pain was induced with a 15 s thermal stimulus at a temperature which reproduced the same pain intensity as the 15 s PA.
Interventions: Anesthesia was induced with a combination of etomidate and fentanyl; pancuronium bromide was given for muscle relaxation; and anesthesia was maintained with isoflurane, desflurane, or sevoflurane in 100% oxygen.
From the fifth week, humid air was induced with a salt-saturated solution.
(a1) Five micrometers was induced with a negative DEP force to be concentrated in the middle area.
Anesthesia was induced with a 1-min infusion of 0.5 mg/kg propofol followed by 10-mg boluses of propofol every 30 s until loss of consciousness.
Experimental ARDS was induced with a previously described double-hit model [17], consisting in warm saline solution lung lavages followed by injurious mechanical ventilation.
The length of the additional tube was titrated until respiratory acidosis was induced with a target pH value ranging between 7.0 and 7.1.
Once the optical density at 600 nm reached 0.6, the culture was induced with a 0.8 mM final concentration of isopropyl-β-D-thiogalactopyranoside and incubated for 20 h at 20°C in shaking incubator at 150 rpm.
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