To study more precisely the effect of Notch expression in MCF-7 cells, we generated N1ICD-inducible clones using the TetOFF system, so that gene expression was induced when the antibiotic doxycycline (DOXY) was removed from the culture medium.
Oddly, the study found that less stress was induced when checking work e-mail rather than other online interactions.
In contrast, an increased force was induced when a negative response occurred.
A protection of up to 98% was induced when KETc1 was used as a chimera fused to BLS.
We showed here that miR172d levels were increased in the leaves as a plant aged and that flowering was induced when that gene was over-expressed.
However, potent electromagnetic interference on electrocardiogram (ECG) recording was induced when the MyLink loop antenna was placed on top or near the ECG electrodes.
Tracheary element formation was induced when calli were subcultured several times on maintenance medium and subsequently transferred to solidified Murashige and Skoog medium (Murashige and Skoog [1962]) supplemented with 4 g L−1 activated charcoal.
At −70 °C the orthorhombic cubic phase transition was induced when the loading pressure was 5 GPa and the loading time was 1 ns, whereas the orthorhombic tetragonal cubic phase transition occurred when the loading time increased to 10 μs.
Results demonstrated that the maximum stress was induced when the stent force was 8-times higher than a standard commercially available stent-graft and this represented about 20% of the failure strength of the UBM material.
Investigation of different induction strategies revealed that induction was optimal when the temperature was maintained at 30 °C, the inducer (lactose) was fed at a rate of 0.2 g L−1 h−1, and protein expression was induced when the cell density (OD600) reached 50.
In the bioreactor, a biomass of 17.5 g L−1 and an ARA yield of 2.7 g L−1 were achieved when using sole yeast extract, while cell autolysis was induced when using sole corn steep liquor; When using the combined nitrogen source with corn steep liquor and yeast extract at a 3 7 weight ratio, the biomass and ARA yield were significantly improved to 37 and 7.8 g L−1, respectively.
