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Expression of the nascent chain was induced for 1 h by adding 0.2% arabinose.
The nascent chain was induced for 1 h by adding 0.2% arabinose.
For Int complementation, ectopic Int was induced for 20 min prior to phage infection.
Liver ischemia was induced for 45 min then reperfusion was allowed for 1 h.
Spinal cord ischemia was induced for 25 min in male Sprague-Dawley rats.
In two groups of 8 dogs each, ventricular fibrillation was induced for 60 and 90 minutes, respectively, while the dogs were on bypass.
In the remaining culture the degradation of Xrn1 was induced for 30 min by adding auxin to a final concentration of 0.5 mM.
Upon transduction, cells were selected with Puromycin for one passage, after which dCas9-cat3a was induced for two passages followed by genomic DNA extraction and whole-genome bisulfite sequencing (WGBS).
mAID-nanobodies expression in Venus-LMNA cells was induced for 24 h by 10 20 ng/ml tetracycline and by 1 μg/ml tetracycline for CCND1-Venus or Venus cells unless otherwise indicated.
Five minutes after return of spontaneous circulation, cardiac arrest was induced for 1 min, with the CPR protocol following as described above.
During each period milk fat depression was induced for 10 d by feeding a low-fiber, high-UFA diet [25.9% neutral detergent fiber (NDF) and 3.3% C18 2].
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