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The cavity was immediately washed with sterile PBS.
Upon removal, tail tissue was immediately washed with ice-cold 1× PBS.
To prevent thermal damage to the ileum, the section was immediately washed with normal saline.
The filter was immediately washed twice with 3 ml ice-cold solution containing 100 mM KCl, 2 mM EGTA, and 40 mM imidazole (pH 7.0).
The biomass was immediately washed with 20 ml sterile, demineralised water, after which the biomass was removed to liquid nitrogen and stored at -80°C for further analysis.
After electrophoresis, the gel was immediately washed by five successive 30-min washes in 0.2 M cold glycine-NaOH buffer (pH 8.0) with first two washes contained 25% (v/v) isopropanol to remove the SDS.
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Well, it doesn't help that everything they put up is immediately washed away by a tropical storm because it's monsoon season.
The fish were immediately washed, gutted, filleted and de-skinned.
After electrophoresis, gels were immediately washed with distilled water and activity staining of bacterial PO was conducted in 100 mM NAc buffer (pH 5) containing different PO substrates such as 2,6-DMP, ABTS, guaiacol and para-phenylenediamine.
The extracted teeth were immediately washed under running water and placed in formaldehyde solution diluted to a 1 10 ratio with distilled water and stored in this manner for a minimum of 5 days to allow fixation.
After the incubation period, the cells were immediately washed three times in cold RPMI/10% FCS.
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